Miniscope V4
Founding developer Daniel Aharoni
$1,850.00 USD
The V4 is a leap forward in miniscope technology. It will serve as the platform for a new generation of miniscopes for years to come. This high-end solution will help to advance and disseminate miniature microscopy to the fast increasing open-source neuroscience community.
Miniscope V4 uses wide-field fluorescence imaging to record neural activity in awake, freely moving mice. V4 miniscopes from LabMaker come fully-assembled, tested, and ready to use. It is compatible with the Data Acquisition System (DAQ) with V4 firmware.
KEY FEATURES
- >1mm diameter field of view
- ~1mm working distance
- with electronic focal adjustment of +/-200um with electrowetting lens; no more mechanical sliding in order to focus
- all high resolution achromatic optics (no internal GRIN lens)
- 2.6 grams, 22 mm tall
- absolute head orientation sensor
- requires ~1/5th the excitation power of previous systems
- still uses only a single coaxial cable (down to 0.3mm in diameter) for power, communication, and data.
CONTENTS
- Fully-assembled and tested Miniscope V4
- Lens configuration 1
- Corning (Varioptics) 2.5mm clear aperture electrowetting lens
- aluminium objective sleeve for easy, repeated mounting (for base plate variant 2)
- one separate base plate variant 2 with screw
- 300 cm coaxial cable, 1 mm diameter, Hirose U.FL connector
FULFILLMENT
The current lead-time of new orders for Miniscope V4 is 2-3 weeks from the date of ordering.
USING A MINISCOPE
is straightforward when you follow the detailed UCLA step-by-step instructions. (requires prior registration with miniscope.org)
Experimental timeline (by Denise Cai)
- A-100 Miniscope recording on a linear track
- A-110 Imaging GCamp6F in CA1 using the miniscope
- B-100 Imaging a test slide expressing TetTag GFP
- B-110 Miniscope baseplate preparation
- B-120 Miniscope baseplate surgery
- B-130 Handling the animal for miniscope attachment
- B-140 Attaching the miniscope
- B-150 Removing the miniscope
- B-160 Setting miniscope focus height
- PP - GRIN lens implantation surgery
- PP - Image processing and analysis
- Workshop: Imaging Principles and Microscope Design (5.1GB)
- Working with animal behavior [login to miniscope.org beforehand]
PUBLICATION
Zhe Dong, William Mau, Yu (Susie) Feng, Zachary T. Pennington, Lingxuan Chen, Yosif Zaki, Kanaka Rajan, Tristan Shuman, Daniel Aharoni, Denise J. Cai (2021). Minian: An open-source miniscope analysis pipeline. BioRxiv.
