Mini2P Implant
Founding developers Edvard Moser, May-Britt Moser, and Weijian Zong
$149.00 USD
The Mini2P is a 2-photon miniscope for large-scale calcium imaging in freely moving mice. It enables stable simultaneous recordings of over a thousand cells across multiple planes of densely active cortical regions during a wide range of behavioral tasks without affecting the animal's behavior.
EXPERIMENTAL CAPABILITIES
The experimental setup involves attaching a custom-made titanium head-bar to the skull and cannula. This combination of the cannula, head-bar, and skull forms a stable and rigid frame for positioning the scope.
Contact LABmaker for different assemblies, e.g. involving a GRIN lens or longer prism.
The different variants of Mini2P implants currently offered are:
1. Prism 1.6x1.3 Assembly
- Implant for MEC, RSC, PFC, and vertical view of neocortex
- Compatible with D0277 Objective Lens
2. Plug 1.8x1.5 Assembly with Cannula
- Implant for CA1 imaging
- Compatible with D0277 Objective Lens
3. Plug 1.8x1.5 Assembly w/o Cannula
- Implant for neocortex imaging
- Compatible with D0277 Objective Lens
4. Coverslip 5x0.15 Assembly
- Implant for neocortex imaging
- Compatible with D0254 Objective Lens
5. Coverglass 4.6x1.9 Assembly
- Implant for neocortex imaging
- Compatible with D0277 Objective Lens
KEY FEATURES
1. Prism 1.6x1.3 Assembly
- Prism 1.6x1.3 + Coverslip 5x0.15 + Cannula 5.1x2
- Glass + AR coating
- FOV change range: 1.3 mm
2. Plug 1.8x1.5 Assembly with Cannula
- Plug 1.8x1.5 + Coverslip 5x0.15 + Cannula 5.1x2
- Glass + AR coating
- FOV change range: 1.5 mm
3. Plug 1.8x1.5 Assembly w/o Cannula
- Plug 1.8x1.5 + Coverslip 5x0.15
- Glass + AR coating
- FOV change range: 1.5 mm
4. Coverglass 5x0.15 Assembly
- Coverglass 4.6x1.9 + Coverslip 5x0.15 + Cannula 5.1x2
- Glass + AR coating
- FOV change range: 4 mm
5. Coverslip 4.6x1.9 Assembly
- Coverslip 5x0.15 + Cannula 5.1x2
- AR coating
- Larger field of view
- FOV change range: 5mm
CONTENTS
- Assembled implant
PUBLICATION
W Zong, HA Obenhaus, ER Skytøen, H Eneqvist, NL de Jong, R Vale, MR Jorge, MB Moser, EI Moser (2022). Large-scale two-photon calcium imaging in freely moving mice. Cell.
