The OptoPlate-96 permits high-throughput three-color optogenetics experiments with simultaneous manipulation of red- and blue-light-sensitive optogenetic probes. Its design prevents cross-illumination between microwells in 96-well plates. An active cooling system minimizes sample heating during light-intensive experiments. Each well position can also illuminate 4-wells of a 384-well plate.
Multi-colour optogenetics provides a method for analysing multi-pathway cell networks in a systematic manner.
LJ Bugaj & WA Lim (2019). High-throughput multicolor optogenetics in microwell plates. Nature Protocols, 14, 2205–2228.
Thomas, OS, Hörner, M & Weber, W (2020). A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96. Nature Protocols.