Mini2P Bundle: 2-Photon Microscope

Versatile and High-Precision Imaging

The Mini2P is a cutting-edge 2-photon miniscope designed for advanced large-scale calcium imaging in freely moving mice. This compact yet powerful device allows for stable, simultaneous recordings of several hundred cells across multiple planes, capturing densely active regions with exceptional clarity. Ideal for a wide range of behavioral tasks, the Mini2P ensures precise imaging without disrupting the animal's natural behavior, making it an invaluable tool for neuroscience research.

EXPERIMENTAL CAPABILITIES

The 'Mini2P Bundle' is a fully equipped 2-photon miniscope system that is ready to scan including the computer with all necessary hardware. It can be delivered with or without a 920 nm laser. This setup is perfect for labs ready to start with 2-photon imaging who want to avoid obtaining Mini2P parts individually. It allows for large-scale, high-speed neuronal imaging across multiple planes, with the capability to image over 1,000 neurons in freely moving animals. Its adaptable, lightweight design supports a variety of behavioral tasks such as navigation and social interaction, while maintaining image stability. The system is easily integrated with other recording and stimulation technologies for versatile research applications.

KEY FEATURES

• Weighing only 2.6 g with SiPM, the scope allows for free movement and natural behavior in animals
• Large-angle MEMS FOV: 0.5x0.5 mm² or Fast MEMS FOV: 0.42x0.42 mm²
• 15 Hz frame rate at 256 x 256 pixels for dynamic recording with a large-angle MEMS or 40 Hz with Fast MEMS
• Unparalleled deep tissue visualization, with or without implants like GRIN lenses or prisms. The advanced integrated µTlens technology provides precise focal adjustments across a 240 µm range.
  
• All components optimized for freely behaving animals, supporting long-term studies and complex behavioral experiments

CONTENTS 

• Mini2P Scope
  
• Mini2P SiPM Detector
• Mini2P Scope Cable Harness
• Mini2P Control Box
  
• Mini2P Sacrificial Connector (Pack of 3)
• Mini2P Headbar (Pack of 5)
• Mini2P Implant (Pack of 5)
• Mini2P Dummy Scope (Pack of 10)
• Mini2P Spacer-50 (Pack of 25)
• Mini2P Base Plate (Pack of 5)
• Mini2P Adapter (Pack of 5)
• Piezo driver
• Control computer
• Control PCIexpress card vDAQ
  
• Control software: ScanImage Basic (MATLAB not included)
• 2-photon Spark Laser ALCOR 920-Mini2P (optional)

Important Information Regarding the Excitation Fiber:

Please note that the Excitation Fiber is not included in the Mini2P Bundle (without laser) variant.

If you purchase the Mini2P Bundle (with laser) variant, the Excitation Fiber is automatically included with the laser.

For users who already own a Spark laser, the compatible Mini2P Excitation Fiber is available for purchase separately. Please click here to learn more.

If you have a 920 nm 2-photon laser of any kind, we also offer coupling solutions customized to your specific requirements. Please contact us for more details.

PUBLICATION

W Zong, HA Obenhaus, ER Skytøen, H Eneqvist, NL de Jong, R Vale, MR Jorge, MB Moser, EI Moser (2022). Large-scale two-photon calcium imaging in freely moving mice. Cell.

Documentation Source Code Forum