Mini2P Bundle

Versatile and High-Precision Imaging

The Mini2P is a miniature two-photon miniscope designed for advanced large-scale calcium imaging in freely moving mice. This compact yet powerful device enables stable, simultaneous recordings of several hundred cells across multiple planes, capturing densely active regions with exceptional clarity. Ideal for a wide range of behavioral tasks, the Mini2P provides precise imaging without disrupting the animal's natural behavior, making it an invaluable tool for neuroscience research.

The Mini2P Bundle is a fully equipped 2-photon miniscope system that is ready for scanning, including the computer and all necessary hardware. It can be delivered with or without a 920 nm laser. This setup is ideal for labs ready to start with 2-photon imaging and looking to avoid sourcing Mini2P components individually. It enables large-scale, high-speed neuronal imaging across multiple planes, with the capability to image over 1,000 neurons in freely moving animals. Its adaptable, lightweight design supports a variety of behavioral tasks, such as navigation and social interaction, while maintaining image stability. The system can also be easily integrated with other recording and stimulation technologies for versatile research applications.

The bundle now uses MBF Mini vDAQ™ as its standard acquisition and control platform, optimized for Mini2P. Choose Large-angle MEMS for a larger field of view or Fast MEMS for higher frame rates. Both scanner configurations work with Mini vDAQ, with no changes to the Mini2P scope's optical specifications.

• Weighing only 2.6 g with mounted SiPM detectors, the scope allows for free movement and natural behavior in animals
• Large-angle MEMS FOV: 0.5 × 0.5 mm² or Fast MEMS FOV: 0.42 × 0.42 mm²
• 15 Hz frame rate at 256 × 256 pixels with Large-angle MEMS or 40 Hz with Fast MEMS
• Unparalleled deep-tissue visualization, with or without implants such as GRIN lenses or prisms. The advanced integrated µTlens technology provides precise focal adjustments across a 240 µm range.
• Two high-speed acquisition channels with up to 125 MHz sampling and 14-bit digitization (Mini vDAQ)
• Individually characterized, assembled, and tested by LABmaker
• All components are optimized for freely behaving animals, supporting long-term studies and complex behavioral experiments

• Mini2P Scope with Large-angle or Fast MEMS
• Mini2P SiPM detectors, including amplifier electronics and power supply
• Mini2P Scope Cable Harness and system connection cables
• Mini2P Control Box
• µTlens driver
• MBF Mini vDAQ™ acquisition and control hardware, including PCIe card and breakout box
• Compatible control computer
• ScanImage Basic control software (MATLAB not included)
• Mini2P Headbar (pack of 5)
• Mini2P Implant (pack of 5)
• Mini2P Dummy Scope (pack of 10)
• Mini2P Spacer-50 (pack of 25)
• Mini2P Base Plate (pack of 5)
• Mini2P Adapter (pack of 5)
• 2-photon Spark Laser ALCOR 920-Mini2P (optional)

Important Information Regarding the Excitation Fiber:

Please note that the Excitation Fiber is not included in the Mini2P Bundle (without laser) variant.

If you purchase the Mini2P Bundle (with laser) variant, the Excitation Fiber is automatically included with the laser.

For users who already own a Spark laser, the compatible Mini2P Excitation Fiber is available for purchase separately.

If you have a 920 nm 2-photon laser of any kind, we also offer customized coupling solutions to meet your specific requirements. Please contact us for more details.

Note: For experiments requiring a greater number of input/output connections, LABmaker also offers the Mini2P Bundle with a Full vDAQ™ configuration. If you are interested, please contact us for a customized price quotation.

W Zong, HA Obenhaus, ER Skytøen, H Eneqvist, NL de Jong, R Vale, MR Jorge, MB Moser, EI Moser (2022). Large-scale two-photon calcium imaging in freely moving mice. Cell.