OptoPlate-96 Bugaj Lab

Founding developer Lukasz Bugaj

Accessories for OptoPlate-96 Bugaj Lab

Founding developer Lukasz Bugaj

Lukasz Bugaj works at the Department of Bioengineering, University of Pennsylvania. His research focus is on the mechanisms that govern essential cell decisions like growth, differentiation, and death.

The OptoPlate-96 permits high-throughput three-color optogenetics experiments with simultaneous manipulation of red- and blue-light-sensitive optogenetic probes. Its design prevents cross-illumination between microwells in 96-well plates. An active cooling system minimizes sample heating during light-intensive experiments. Each well position can also illuminate 4-wells of a 384-well plate.


Multi-colour optogenetics provides a method for analysing multi-pathway cell networks in a systematic manner.

  • Immunofluorescence analysis.
  • 96- or 384-well plate illumination.
  • Three colour combinations that can stimulate the common mammalian cell optogenetics probes.


  • Illumination is programmable and can be applied remotely.
  • Offer data-rich experiments with precise perturbations.
  • Multiple stimulation colours, and the potential for programmable high-throughput analysis.
  • Independent control of stimulation colours, intensity, dynamics, and time points.
  • Arduino microcontroller to store and execute user-defined illumination profile.


  • Light isolated lid
  • 96 or 384 well plate
  • OptoPlate illuminator
  • Thermal pad
  • Aluminium plate
  • Heat sink
  • Fan
  • Base plate
  • Power supplies (US only)


Shipment will be done in 6-8 weeks from ordering.


LJ Bugaj & WA Lim (2019). High-throughput multicolor optogenetics in microwell plates. Nature Protocols, 142205–2228.

Thomas, OS, Hörner, M & Weber, W (2020). A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96. Nature Protocols

Documentation  Source Code

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