Miniscope - Complete set of components

Founding developers Daniel Aharoni, Peyman Golshani, Alcino Silva, and Baljit Khakh

 
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Add-ons for Miniscope - Complete set of components

Founding developers Daniel Aharoni, Peyman Golshani, Alcino Silva, and Baljit Khakh

The Golshani, Silva, and Khakh Labs at the University of California, Los Angeles, with Daniel Aharoni as main coordinator collaborated in the development of the Miniscope. Its design was pioneered by Mark Schnitzer's Lab at Stanford and published in Nature Methods in 2011. The UCLA miniscope community maintains a WiKi to help disseminate this open source technology to the larger neuroscience community.

A Miniscope uses wide-field fluorescence imaging to record neural activity in awake, freely moving mice. It has a mass of 3 grams and uses a single, flexible coaxial cable for power, control signals, and imaging data. Using this system, it is possible to image the hippocampal CA1, subiculum, visual cortex and other areas using a GRIN lens.

 The set for a fully functional unit contains:

  • CMOS imaging sensor PCB presoldered to an excitation LED PCB and a COAX cable
  • COAX cable extension (1m)
  • Housing containing a dichroic mirror, an emission filter, an excitation filter and an achromatic lens
  • Half ball lens
  • Focusing slider with one set screw
  • Stainless steel thread-forming screws for thin plastic (x6)
  • TORX T1 screwdriver
  • Hex wrench – 0.70mm
  • Base plate with set screw
  • GRIN lens by Grintech, 1.8/4.2 mm, NA0.5, pitch 0.25, 4th order index of refraction profile, bio-compatible glass
  • Metal sleeve for thin GRIN lens (Relay lens imaging)
  • Assembly does not require soldering

NOTE: From now on, the Miniscope components set comes with an updated coaxial cable and extension. The new cable diameter has been reduced to just 1.13mm, in replacement of the previous version with 2.80mm. This allows more flexibility in the movement and reduces the stress on the wire.

For imaging with the Miniscope you need a Data Acquisition Controller connected via USB 3.0 to a computer with the UCLA data acquisition software installed.

 

Using a miniscope

is straightforward when you follow the detailed UCLA step-by-step instructions. (requires prior registration with miniscope.org)

Experimental timeline (by Denise Cai)

Documentation Source Code

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