A Miniscope uses wide-field fluorescence imaging to record neural activity in awake, freely moving mice. It has a mass of 3 grams and uses a single, flexible coaxial cable for power, control signals, and imaging data. Using this system, it is possible to image the hippocampal CA1, subiculum, visual cortex and other areas using a GRIN lens.
The set for a fully functional unit contains:
- CMOS imaging sensor PCB presoldered to an excitation LED PCB and a COAX cable
- COAX cable extension (1m)
- Housing containing a dichroic mirror, an emission filter, an excitation filter and an achromatic lens
- Half ball lens
- Focusing slider with one set screw
- Stainless steel thread-forming screws for thin plastic (x6)
- TORX T1 screwdriver
- Hex wrench – 0.70mm
- Base plate with set screw
- GRIN lens by Grintech, 1.8/4.2 mm, NA0.5, pitch 0.25, 4th order index of refraction profile, bio-compatible glass.
- Assembly does not require soldering
Shipment currently within 2-4 weeks after placing order.
For imaging with the Miniscope you need a Data Acquisition Controller connected via USB 3.0 to a computer with the UCLA data acquisition software installed.
Using a miniscope
is straightforward when you follow the detailed UCLA step-by-step instructions. (requires prior registration with miniscope.org)
Experimental timeline (by Denise Cai)
Documentation Source Code