For instructions on how to operate Chi.Bio please see the Operation Manual
Chi.Bio is an all-in-one experimental automation device for characterisation and manipulation of biological systems. Its broad feature set allows researchers to perform and automate many common experimental protocols from synthetic, systems, and evolutionary biology, without requiring any additional laboratory hardware. It can operate as a Turbidosotat or Chemostat to regulate cell growth, whilst providing heating, stirring, measurement of multiple fluorescence proteins, and optogenetic/UV/daylight inputs.
By combining regulation of biological parameters of interest and accurate measurement into a single platform Chi.Bio facilitates reliable and repeatable experimental procedures, which have long been recognised as a major challenge for biological science research.
Up to eight Chi.Bio reactor units can interface with each control computer, which runs the platform’s open-source operating system (developed in Python). A web interface (accessible on a connected PC or network) plots data in real-time as it is collected, and allows users to change and control ongoing experiments.
The set includes USB cables and a power supply but does not include silicone tubing. Details about which ones to use can be found on the hardware page of the official Chi.Bio website. You can purchase additional reactors and pump sets as add-ons in packs of 4 or 7.
• Harrison Steel, Robert Habgood, Ciarán Kelly, Antonis Papachristodoulou. Chi.Bio: An open-source automated experimental platform for biological science research. BioRxiv.
• Harrison Steel, Robert Habgood, Ciarán Kelly, Antonis Papachristodoulou. In situ characterisation and manipulation of biological systems with Chi.Bio. PLoS Biol 18(7)
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