The V4 is a leap forward in miniscope technology. It will serve as the platform for a new generation of miniscopes for years to come. This high-end solution will help to advance and disseminate miniature microscopy to the fast increasing open-source neuroscience community.
Significant contributors to the V4 were also the Cai and Shuman labs at Mount Sinai, and the Blair lab at UCLA. It builds on the Golshani, Silva, and Khakh UCLA labs' initiative coordinated by Daniel Aharoni of making Mark Schnitzer's pioneering invention (Nature Methods 2011) open source.
Miniscope V4 uses wide-field fluorescence imaging to record neural activity in awake, freely moving mice. The Varioptics electrowetting lens allows electronic focussing. V4 miniscopes from LabMaker come fully-assembled, tested, and ready to use.
New orders can be shipped out in 6-8 weeks from the date of ordering.
is straightforward when you follow the detailed UCLA step-by-step instructions. (requires prior registration with miniscope.org)
Experimental timeline (by Denise Cai)
Zhe Dong, William Mau, Yu (Susie) Feng, Zachary T. Pennington, Lingxuan Chen, Yosif Zaki, Kanaka Rajan, Tristan Shuman, Daniel Aharoni, Denise J. Cai (2021). Minian: An open-source miniscope analysis pipeline. BioRxiv.